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Image Search Results
Journal: Nature Communications
Article Title: Sialic acid catabolism drives intestinal inflammation and microbial dysbiosis in mice
doi: 10.1038/ncomms9141
Figure Lengend Snippet: ( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. thetaiotaomicron . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
Article Snippet: E. coli (EHV2) and
Techniques: Bacteria, Cell Culture, Expressing, Flow Cytometry, Fluorescence, Comparison, Control