statistics toolbox release 2016 b Search Results


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LI-COR 6400 portable photosynthesis system
6400 Portable Photosynthesis System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSMZ b thetaiotaomicron
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
B Thetaiotaomicron, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FOSS GmbH bedtools intersectbed command
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
Bedtools Intersectbed Command, supplied by FOSS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genomatica inc 1,4-bdo production
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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DSMZ b eggerthii dsm 20697
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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90
DSMZ b marina dsm 3645
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
B Marina Dsm 3645, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
DSMZ b intestinalis dsm 17393
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
B Intestinalis Dsm 17393, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
MathWorks Inc matlab 2016b
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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86
Abbott Laboratories ligo virgo signals
( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. <t>thetaiotaomicron</t> . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).
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Image Search Results


( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. thetaiotaomicron . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).

Journal: Nature Communications

Article Title: Sialic acid catabolism drives intestinal inflammation and microbial dysbiosis in mice

doi: 10.1038/ncomms9141

Figure Lengend Snippet: ( a ) In stimulation assay, bacteria were fixed with 0.5% paraformaldehyde in PBS, washed and co-cultured at a ratio of 100:1 with mouse mesenteric CD11c + DCs. Ec, E. coli EHV2; Bt, B. thetaiotaomicron . Cell surface expression of CD86, CD40 and MHC-II was analysed by flow cytometry. ( b ) Human monocytic THP-1 cells were stimulated with fixed bacteria at ratios of 5:1 and 1:1 to cells for 14 h at 37 °C. Cell surface expression of CD54 was analysed by flow cytometry. In ( a , b ), data are shown as mean fluorescence intensity, MFI±s.e.m. from three independent experiments. N =6, * P <0.05 (ANOVA, Bonferroni's multiple comparison test). ( c ) Cytokine expression in the culture supernatant of stimulated mesenteric CD11c + DCs stimulated for 14 h at 37 °C with fixed Ec, Bt and 500 ng ml −1 of LPS. PBS was used as negative stimulation control. Data are shown as mean±s.e.m. from two independent experiments. N =4–5, * P <0.05 (ANOVA, Bonferroni's multiple comparison test).

Article Snippet: E. coli (EHV2) and B. thetaiotaomicron (DSMZ 2079T) were fixed in 0.5% paraformaldehyde for 15 min at room temperature and washed with PBS before stimulation.

Techniques: Bacteria, Cell Culture, Expressing, Flow Cytometry, Fluorescence, Comparison, Control